CONCENTRATED ANTINEURITIC VITAMIN PREPARED FROM BREWERS' YEAST
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چکیده
منابع مشابه
The Differential Extraction from Dried Brewers’ Yeast of the Antineuritic (vita-
Since the demonstration of the dual nature of vitamin B in 1926 (1, 2) which has been amply confirmed in recent years (35), there has been no satisfactory method for the separation of the heat-labile antineuritic (vitamin B,) from the more heat-stable growth-promoting (vitamin B,) component of the vitamin B complex. There are numerous experiments recorded in the lit,erature indicating that at l...
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Choline kinase was purified approximately 300-fold, in 5% yield, from an autolysate of dried brewers’ yeast. A molecular weight of 67,000 was estimated using a Stokes radius of 33 A, as determined by Sephadex G-200 chromatography. An s~,,,~ of 4.8 S was obtained by sucrose density gradient centrifugation. Enzyme activity was diminished by sulfhydryl inhibitors and stabilized by the presence of ...
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In a program to elucidate the mechanism by which phosphoglucose isomerasel participates in the catalyzed isomerization between glucose 6-phosphate and fructose g-phosphate, isolation of the enzyme from several sources is pursued as the basis for quantitative studies of its protein nature. The comparative investigation of several heteroenzymes appears to be a valuable tool in identifying the str...
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THIS work is being communicated now for two reasons. Confirmation of a previous statement is needed that preparations of activity under 0.1 mg. per day pigeon dose can be obtained from yeast. Moreover the details of preparing these more active concentrates are required in explanation of an accompanying paper [Peters, Kinnersley, Orr-Ewing and Reader, 1928]. It must be stated at the outset that ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1926
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)84694-4